Prerna Soni ¹, A. N. Bahadur ², U. Tiwari ², V. K. Kanungo ²

Affiliations
1 Department of Botany and Biotechnology, Laboratory of Plant Tissue Culture, Government E. Raghvendra Rao P. G. College of Science, Bilaspur - 495006, Chhattisgarh, IN
2 Government Nagarjuna, P. G. College of science, Raipur - 492010, Chhattisgarh, IN

Abstract

Withania somnifera, a popular Indian medicinal plant has long been used commonly in ayurvedic system of medicine. All the parts of the plants have shown remarkable importance in the field of pharmacology. The present paper gives an account of information on in-vitro micro propagation of medicinally important plant for the generation of multiple shoots and ischolar_mains of Withania somnifera and its phyto-chemical activities. Shoots were induced from the axillary buds of Withania somnifera on Murashige and Skoogs's (MS) basal media supplemented with different concentrations of BAP (0.10mg/l, 0.25mg/l, 0.50mg/l, 0.75mg/l, 1.00mg/l). Axillary bud explants showed initiation of shoots with the concentrations of 1mg/l and it is found to be most effective for multiple shoot generation within minimum time period. The leaf, stem and ischolar_main of Withania somnifera were analyzed for phyto-chemical screening. The micro propagated plants were transplanted to pots for hardening.

Keywords

In-vitro Multiplication, MS Medium, Withania somnifera, Shoot Bud Culture, Benzyl Amino Purine, Axiliary

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